Visualizing 3D structure and dynamics at the molecular scale is a current and critical need in biomedical research. Many sub-cellular features, for example the morphology of many organelles or the 3D organization of chromatin, cannot be resolved by conventional light microscopy.
Improving the resolution of light microscopy has therefore been an urgent need in biological research for many decades. Today, several methods achieve sub-100 nm resolution by taking advantage of reversible or irreversible photo-physical switching properties of fluorescent markers.
In our lab we evolve these methods, pushing the temporal and spatial resolution of fluorescence microscopy far beyond the limits of conventional light microscopy.
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